Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	G protein-coupled receptor kinase 6
Gene Symbol:	GRK6
Synonyms:	GPRK6
Gene ID:	2870
UniProt ID:	P43250
Clone ID:	24GB4870
Immunogen:	A synthesized peptide derived from human GRK6
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.

Images

	Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.