Description

Reactivity:	Human, Mouse, Rat
Applications:	WB
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	eukaryotic translation initiation factor 3 subunit B
Gene Symbol:	EIF3B
Synonyms:	PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
Gene ID:	8662
UniProt ID:	P55884
Clone ID:	24GB4545
Immunogen:	A synthesized peptide derived from human eIF3B
Dilution:	WB 1:1,000-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in cytoplasmic stress granule. Part of eukaryotic translation initiation factor 3 complex.

Images

Western blotting analysis using anti-EIF3B antibody (Cat#63215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF3B, eukaryotic translation initiation factor 3 subunit B.

Western blotting analysis using anti-EIF3B antibody (Cat#63215). EIF3B expression in wild type (WT) and EIF3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

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