Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	RAB5A, member RAS oncogene family
Gene Symbol:	RAB5A
Synonyms:	RAB5
Gene ID:	5868
UniProt ID:	P20339
Clone ID:	24GB4525
Immunogen:	A synthesized peptide derived from human Rab5
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton.

Images

	Western blotting analysis using anti-RAB5A antibody (Cat#63208). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB5A antibody (Cat#63208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-RAB5A antibody (Cat#63208). RAB5A expression in wild-type (WT) and RAB5A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAB5A antibody (Cat#63208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of RAB5A expression in HepG2 cells using anti-RAB5A antibody (Cat#63208, 1:2,000). Green, isotype control; red, RAB5A.