Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	presenilin 1
Gene Symbol:	PSEN1
Synonyms:	AD3; FAD; PS1; PS-1; S182; CMD1U; PSNL1; ACNINV3
Gene ID:	5663
UniProt ID:	P49768
Clone ID:	24GB4510
Immunogen:	A synthesized peptide derived from human Phospho-Presenilin 1 (Ser310)
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Alzheimer’s disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.

Images

	Western blotting analysis using anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205). Phospho-presenilin 1 (Ser310) expression in wild-type (WT) and PSEN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PSEN1, presenilin 1.
	Flow cytometric analysis of Phospho-Presenilin 1 (Ser310) expression in HeLa cells using anti-Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:2,000). Green, isotype control; red, Phospho-Presenilin 1 (Ser310).
	Immunocytochemical staining of Hela cells with Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Presenilin 1 (Ser310) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Scale bar, 20 μm.