Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	basigin (Ok blood group)
Gene Symbol:	BSG
Synonyms:	OK; 5F7; TCSF; CD147; EMPRIN; HAb18G; EMMPRIN
Gene ID:	682
UniProt ID:	P35613
Clone ID:	24GB4260
Immunogen:	A synthesized peptide derived from human CD147
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-CD147 antibody (Cat#63151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD147 antibody (Cat#63151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-CD147 antibody (Cat#63151). CD147 expression in wild-type (WT) and BSG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CD147 antibody (Cat#63151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CD147 expression in HepG2 cells using anti-CD147 antibody (Cat#63151, 1:2,000). Green, isotype control; red, CD147.
	Immunocytochemical staining of HepG2 cells with anti-CD147 antibody (Cat#63151, 1:1,000). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.