Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	serpin family E member 1
Gene Symbol:	SERPINE1
Synonyms:	PAI; PAI1; PAI-1; PLANH1
Gene ID:	5054
UniProt ID:	P05121
Clone ID:	24GB4055
Immunogen:	A synthesized peptide derived from human PAI1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

Images

	Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Serpin family E member 1expression in wild-type (WT) and serpin family E member 1 (SERPINE1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Serpin family E member 1 expression in HT-1080 cells using anti-Serpin family E member 1 antibody (Cat#63098, 1:2,000). Green, isotype control; red, Serpin family E member 1.
	Immunocytochemical staining of HT-1080 cells with anti-Serpin family E member 1 antibody (Cat#63098, 1:1,000). Nuclei were stained blue with DAPI; Serpin family E member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.