Description

Reactivity:	Human
Applications:	WB
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Polyclonal antibody
Gene Name:	Jun proto-oncogene, AP-1 transcription factor subunit
Gene Symbol:	JUN
Synonyms:	AP1; p39; AP-1; cJUN; c-Jun
Gene ID:	3725
UniProt ID:	P05412
Immunogen:	A synthesized peptide derived from human c-Jun
Dilution:	WB 1:2,500-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Images

Western blotting analysis using anti-C-Jun antibody (Cat#62824). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-C-Jun antibody (Cat#62824, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-C-Jun antibody (Cat#62824). C-Jun expression in wild type (WT) and C-Jun shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-C-Jun antibody (Cat#62824, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

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