Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	VAMP associated protein A
Gene Symbol:	VAPA
Synonyms:	VAP-A; VAP33; VAMP-A; VAP-33; hVAP-33
Gene ID:	9218
UniProt ID:	Q9P0L0
Clone ID:	24GB2580
Immunogen:	A synthesized peptide derived from human VAPA
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Images

	Western blotting analysis using anti-VAPA antibody (Cat#62817). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VAPA antibody (Cat#62817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-VAPA antibody (Cat#62817). VAPA expression in wild type (WT) and VAPA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-VAPA antibody (Cat#62817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of VAPA expression in HepG2 cells using anti-VAPA antibody (Cat#62817, 1:2,000). Green, isotype control; red, VAPA.
	Immunocytochemical staining of HepG2 cells with anti-VAPA antibody (Cat#62817, 1:1,000). Nuclei were stained blue with DAPI; VAPA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.