Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	ubiquitin protein ligase E3 component n-recognin 5
Gene Symbol:	UBR5
Synonyms:	DD5; EDD; HYD; EDD1
Gene ID:	51366
UniProt ID:	O95071
Clone ID:	24GB2540
Immunogen:	A synthesized peptide derived from human EDD
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Images

	Western blotting analysis using anti-UBR5 antibody (Cat#62788). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBR5 antibody (Cat#62788, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-UBR5 antibody (Cat#62788). UBR5 expression in wild type (WT) and UBR5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBR5 antibody (Cat#62788, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of UBR5 expression in HT-1080 cells using anti-UBR5 antibody (Cat#62788, 1:2,000). Green, isotype control; red, UBR5.
	Immunocytochemical staining of HT-1080 cells with anti-UBR5 antibody (Cat#62788, 1:1,000). Nuclei were stained blue with DAPI; UBR5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.