Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Sad1 and UNC84 domain containing 1 |
| Gene Symbol: | SUN1 |
| Synonyms: | UNC84A |
| Gene ID: | 23353 |
| UniProt ID: | O94901 |
| Clone ID: | 24GB2575 |
| Immunogen: | A synthesized peptide derived from human SUN1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the unc-84 homolog family and encodes a nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Alternatively spliced transcript variants have been described.
Images
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Western blotting analysis using anti-SUN1 antibody (Cat#62740). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SUN1 antibody (Cat#62740, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-SUN1 antibody (Cat#62740). SUN1 expression in wild-type (WT) and SUN1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SUN1 antibody (Cat#62740, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of SUN1 expression in HepG2 cells using anti-SUN1 antibody (Cat#62740, 1:2,000). Green, isotype control; red, SUN1. |
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Immunocytochemical staining of H9C2 cells with anti-SUN1 antibody (Cat#62740, 1:1,000). Nuclei were stained blue with DAPI; SUN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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