Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	three prime repair exonuclease 1
Gene Symbol:	TREX1
Synonyms:	CRV; AGS1; DRN3; HERNS; RVCLS
Gene ID:	11277
UniProt ID:	Q9NSU2
Clone ID:	24GB2440
Immunogen:	A synthesized peptide derived from human TREX1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a nuclear protein with 3′ exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-TREX1 antibody (Cat#62730). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TREX1 antibody (Cat#62730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-TREX1 antibody (Cat#62730). TREX1 expression in wild type (WT) and TREX1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TREX1 antibody (Cat#62730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of TREX1 expression in HepG2 cells using anti-TREX1 antibody (Cat#62730, 1:2,000). Green, isotype control; red, TREX1.
	Immunocytochemical staining of HepG2 cells with anti-TREX1 antibody (Cat#62730, 1:1,000). Nuclei were stained blue with DAPI; TREX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.