Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	structural maintenance of chromosomes 4
Gene Symbol:	SMC4
Synonyms:	CAPC; CAP-C; SMC-4; SMC4L1
Gene ID:	10051
UniProt ID:	Q9NTJ3
Clone ID:	24GB2400
Immunogen:	A synthesized peptide derived from human SMC4
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene belongs to the ‘structural maintenance of chromosomes’ (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.

Images

	Western blotting analysis using anti-SMC4 antibody (Cat#62727). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMC4 antibody (Cat#62727, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-SMC4 antibody (Cat#62727). SMC4 expression in wild type (WT) and SMC4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMC4 antibody (Cat#62727, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of SMC4 expression in HT-1080 cells using anti-SMC4 antibody (Cat#62727, 1:2,000). Green, isotype control; red, SMC4.
	Immunocytochemical staining of HT-1080 cells with anti-SMC4 antibody (Cat#62727, 1:1,000). Nuclei were stained blue with DAPI; SMC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.