Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	sorting nexin 5
Gene Symbol:	SNX5
Gene ID:	27131
UniProt ID:	Q9Y5X3
Clone ID:	24GB2310
Immunogen:	A synthesized peptide derived from human SNX5
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein functions in endosomal sorting, the phosphoinositide-signaling pathway, and macropinocytosis. This gene may play a role in the tumorigenesis of papillary thyroid carcinoma. Alternative splicing results in multiple transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Sorting nexin 5 expression in wild type (WT) and Sorting nexin 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Sorting nexin 5 expression in HAP-1 cells using anti-Sorting nexin 5 antibody (Cat#62718, 1:2,000). Green, isotype control; red, Sorting nexin 5.