Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	somatostatin receptor 5
Gene Symbol:	SSTR5
Synonyms:	SST5; SS-5-R
Gene ID:	6755
UniProt ID:	P35346
Clone ID:	24GB2600
Immunogen:	A synthesized peptide derived from human SSTR5
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Somatostatin and its related peptide cortistatin exert multiple biological actions on normal and tumoral tissue targets by interacting with somatostatin receptors (SSTRs). The protein encoded by this gene is one of the SSTRs, which is a multi-pass membrane protein and belongs to the G-protein coupled receptor 1 family. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase, and different regions of this receptor molecule are required for the activation of different signaling pathways. A mutation in this gene results in somatostatin analog resistance. Alternatively spliced transcript variants have been identified in this gene.

Images

	Western blotting analysis using anti-somatostatin receptor 5 antibody (Cat#62679). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-somatostatin receptor 5 antibody (Cat#62679, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Somatostatin receptor 5 antibody (Cat#62679). Somatostatin receptor 5 expression in wild type (WT) and Somatostatin receptor 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Somatostatin receptor 5 antibody (Cat#62679, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Somatostatin receptor 5 expression in HepG2 cells using anti-Somatostatin receptor 5 antibody (Cat#62679, 1:2,000). Green, isotype control; red, Somatostatin receptor 5.
	Immunocytochemical staining of HepG2 cells with anti-Somatostatin receptor 5 antibody (Cat#62679, 1:1,000). Nuclei were stained blue with DAPI; Somatostatin receptor 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.