Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | serine racemase |
| Gene Symbol: | SRR |
| Synonyms: | ILV1; ISO1 |
| Gene ID: | 63826 |
| UniProt ID: | Q9GZT4 |
| Clone ID: | 24GB2345 |
| Immunogen: | A synthesized peptide derived from human Serine racemase |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables several functions, including L-serine ammonia-lyase activity; PDZ domain binding activity; and heterocyclic compound binding activity. Involved in carboxylic acid metabolic process and response to lipopolysaccharide. Located in cytoplasm and neuronal cell body.
Images
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Western blotting analysis using anti-Serine racemase antibody (Cat#62663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Serine racemase antibody (Cat#62663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-Serine racemase antibody (Cat#62663). Serine racemase expression in wild type (WT) and Serine racemase shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Serine racemase antibody (Cat#62663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Serine racemase expression in HepG2 cells using anti-Serine racemase antibody (Cat#62663, 1:2,000). Green, isotype control; red, Serine racemase. |
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Immunocytochemical staining of HepG2 cells with anti-Serine racemase antibody (Cat#62663, 1:1,000). Nuclei were stained blue with DAPI; Serine racemase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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