Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	ubiquitination factor E4B
Gene Symbol:	UBE4B
Synonyms:	E4; UFD2; HDNB1; UBOX3; UFD2A
Gene ID:	10277
UniProt ID:	O95155
Clone ID:	24GB1765
Immunogen:	A synthesized peptide derived from human UBE4B
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Images

	Western blotting analysis using anti-Ubiquitination factor E4 B antibody (Cat#62638). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitination factor E4 B antibody (Cat#62638, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Ubiquitination factor E4 B antibody (Cat#62638). Ubiquitination factor E4 B expression in wild type (WT) and Ubiquitination factor E4 B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Ubiquitination factor E4 B antibody (Cat#62638, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Ubiquitination factor E4 B expression in HepG2 cells using anti-Ubiquitination factor E4 B antibody (Cat#62638, 1:2,000). Green, isotype control; red, Ubiquitination factor E4 B.
	Immunocytochemical staining of HepG2 cells with anti-Ubiquitination factor E4 B antibody (Cat#62638, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.