Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | trafficking from ER to golgi regulator |
| Gene Symbol: | TFG |
| Synonyms: | TF6; HMSNP; SPG57; TRKT3 |
| Gene ID: | 10342 |
| UniProt ID: | Q92734 |
| Clone ID: | 24GB2115 |
| Immunogen: | A synthesized peptide derived from human TRK fused gene |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene.
Images
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Western blotting analysis using anti-TFG antibody (Cat#62630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-TFG antibody (Cat#62630). TFG expression in wild type (WT) and TFG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of TFG expression in HepG2 cells using anti-TFG antibody (Cat#62630, 1:2,000). Green, isotype control; red, TFG. |
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Immunocytochemical staining of HepG2 cells with anti-TFG antibody (Cat#62630, 1:1,000). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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