Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	stomatin like 2
Gene Symbol:	STOML2
Synonyms:	SLP-2; HSPC108
Gene ID:	30968
UniProt ID:	Q9UJZ1
Clone ID:	24GB1870
Immunogen:	A synthesized peptide derived from human Stomatin like 2
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables GTPase binding activity; T cell receptor binding activity; and cardiolipin binding activity. Involved in intracellular calcium ion homeostasis; mitochondrion organization; and protein complex oligomerization. Acts upstream of or within T cell receptor signaling pathway. Located in several cellular components, including immunological synapse; membrane raft; and mitochondrial envelope.

Images

	Western blotting analysis using anti-Stomatin like 2 antibody (Cat#62624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Stomatin like 2 antibody (Cat#62624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Stomatin like 2 antibody (Cat#62624). Stomatin like 2 expression in wild type (WT) and Stomatin like 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-Stomatin like 2 antibody (Cat#62624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Stomatin like 2 expression in HepG2 cells using anti-Stomatin like 2 antibody (Cat#62624, 1:2,000). Green, isotype control; red, Stomatin like 2.
	Immunocytochemical staining of HepG2 cells with anti-Stomatin like 2 antibody (Cat#62624, 1:1,000). Nuclei were stained blue with DAPI; Stomatin like 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.