Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Images

	Western blotting analysis using anti-Peroxiredoxin 4 antibody (Cat#62596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peroxiredoxin 4 antibody (Cat#62596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Peroxiredoxin 4 antibody (Cat#62596). Peroxiredoxin 4 expression in wild type (WT) and Peroxiredoxin 4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Peroxiredoxin 4 antibody (Cat#62596, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Peroxiredoxin 4 expression in HAP-1 cells using anti-Peroxiredoxin 4 antibody (Cat#62596, 1:2,000). Green, isotype control; red, Peroxiredoxin 4.

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