Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	septin 2
Gene Symbol:	SEPTIN2
Synonyms:	DIFF6; NEDD5; SEPT2; NEDD-5; Pnutl3; hNedd5; Septin-2
Gene ID:	4735
UniProt ID:	Q15019
Clone ID:	24GB1525
Immunogen:	A synthesized peptide derived from human Septin 2
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables identical protein binding activity. Predicted to be involved in several processes, including cilium assembly; regulation of exocytosis; and smoothened signaling pathway. Predicted to act upstream of or within regulation of L-glutamate import across plasma membrane and regulation of protein localization. Located in several cellular components, including cytoskeleton; photoreceptor connecting cilium; and sperm annulus. Part of septin complex.

Images

	Western blotting analysis using anti-Septin 2 antibody (Cat#62587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Septin 2 antibody (Cat#62587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-septin 2 antibody (Cat#62587). Septin 2 expression in wild-type (WT) and septin 2 (SEPTIN2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-septin 2 antibody (Cat#62587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Septin 2 expression in C2C12 cells using anti-Septin 2 antibody (Cat#62587, 1:2,000). Green, isotype control; red, Septin 2.