Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | DNA polymerase beta |
| Gene Symbol: | POLB |
| Synonyms: | DNA polymerase beta |
| Gene ID: | 5423 |
| UniProt ID: | P06746 |
| Clone ID: | 24GB1475 |
| Immunogen: | A synthesized peptide derived from human DNA polymerase beta |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Images
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Western blotting analysis using anti-DNA polymerase beta antibody (Cat#62575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA polymerase beta antibody (Cat#62575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-DNA Polymerase beta antibody (Cat#62575). DNA Polymerase beta expression in wild type (WT) and DNA Polymerase beta shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-DNA Polymerase beta antibody (Cat#62575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
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