Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | inorganic pyrophosphatase 1 |
| Gene Symbol: | PPA1 |
| Synonyms: | PP; PP1; IOPPP; HEL-S-66p; SID6-8061 |
| Gene ID: | 5464 |
| UniProt ID: | Q15181 |
| Clone ID: | 24GB1365 |
| Immunogen: | A synthesized peptide derived from human Pyrophosphatase 1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Images
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Western blotting analysis using anti-Pyrophosphatase 1 antibody (Cat#62549). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Pyrophosphatase 1 antibody (Cat#62549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-Pyrophosphatase 1 antibody (Cat#62549). Pyrophosphatase 1 expression in wild type (WT) and Pyrophosphatase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Pyrophosphatase 1 antibody (Cat#62549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Pyrophosphatase 1 expression in HT-1080 cells using anti-Pyrophosphatase 1 antibody (Cat#62549, 1:2,000). Green, isotype control; red, Pyrophosphatase 1. |
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