Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	retinoic acid receptor beta
Gene Symbol:	RARB
Synonyms:	HAP; RRB2; NR1B2; MCOPS12; RARbeta; RARbeta1
Gene ID:	5915
UniProt ID:	P10826
Clone ID:	24GB1045
Immunogen:	A synthesized peptide derived from human Retinoic Acid Receptor beta
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. Alternate promoter usage and differential splicing result in multiple transcript variants.

Images

	Western blotting analysis using anti-Retinoic acid receptor beta antibody (Cat#62480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Retinoic acid receptor beta antibody (Cat#62480, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Retinoic Acid Receptor beta antibody (Cat#62480). Retinoic Acid Receptor beta expression in wild type (WT) and Retinoic Acid Receptor beta shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Retinoic Acid Receptor beta antibody (Cat#62480, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Retinoic acid receptor beta expression in HeLa cells using Retinoic acid receptor beta antibody (Cat#62480, 1:2,000). Green, isotype control; red, Retinoic acid receptor beta.
	Immunocytochemical staining of HeLa cells with anti-Retinoic acid receptor beta antibody (Cat#62480, 1:1,000). Nuclei were stained blue with DAPI; Retinoic acid receptor beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.