Description

Reactivity:	Human, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	peroxiredoxin 6
Gene Symbol:	PRDX6
Synonyms:	PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m
Gene ID:	9588
UniProt ID:	P30041
Clone ID:	24GB910
Immunogen:	A synthesized peptide derived from human PRDX6
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Images

	Western blotting analysis using anti-Peroxiredoxin 6 antibody (Cat#62453). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Peroxiredoxin 6 antibody (Cat#62453). Peroxiredoxin 6 expression in wild type (WT) and Peroxiredoxin 6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Peroxiredoxin 6 expression in HepG2 cells using anti-Peroxiredoxin 6 antibody (Cat#62453, 1:2,000). Green, isotype control; red, Peroxiredoxin 6.
	Immunocytochemical staining of HepG2 cells with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:1,000). Nuclei were stained blue with DAPI; Peroxiredoxin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.