Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	mitogen-activated protein kinase kinase 1
Gene Symbol:	MAP2K1
Synonyms:	MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Gene ID:	5604
UniProt ID:	Q02750
Clone ID:	24GB285
Immunogen:	A synthesized peptide derived from human Phospho-MEK1 (S298)
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Images

	Western blotting analysis using anti-Phospho-MEK1 (S298) antibody (Cat#62318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-MEK1 (S298) antibody (Cat#62318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Phospho-MEK1 (S298) antibody (Cat#62318). Phospho-MEK1 (S298) expression in wild type (WT) and Phospho-MEK1 (S298) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-MEK1 (S298) antibody (Cat#62318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Phospho-MEK1 (S298) expression in C2C12 cells using Phospho-MEK1 (S298) antibody (Cat#62318, 1:2,000). Green, isotype control; red, Phospho-MEK1 (S298).
	Immunocytochemical staining of C2C12 cells with anti-Phospho-MEK1 (S298) antibody (Cat#62318, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.