Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	moesin
Gene Symbol:	MSN
Synonyms:	HEL70; IMD50
Gene ID:	4478
UniProt ID:	P26038
Clone ID:	24GB265
Immunogen:	A synthesized peptide derived from human Moesin
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.

Images

	Western blotting analysis using anti-Moesin antibody (Cat#62313). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Moesin antibody (Cat#62313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Moesin antibody (Cat#62313). Moesin expression in wild type (WT) and Moesin shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Moesin antibody (Cat#62313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Moesin expression in HepG2 cells using Moesin antibody (Cat#62313, 1:2,000). Green, isotype control; red, Moesin.
	Immunocytochemical staining of Hela cells with anti-Moesin antibody (Cat#62313, 1:1,000). Nuclei were stained blue with DAPI; Moesin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.