Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	calcium binding protein 39
Gene Symbol:	CAB39
Synonyms:	MO25; CGI-66
Gene ID:	51719
UniProt ID:	Q9Y376
Clone ID:	25GB3880
Immunogen:	A synthesized peptide derived from human CAB39/MO25
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables kinase binding activity and protein serine/threonine kinase activator activity. Involved in intracellular signal transduction; peptidyl-serine phosphorylation; and positive regulation of protein phosphorylation. Located in extracellular exosome. Part of cytoplasm and serine/threonine protein kinase complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma and pancreatic cancer.

Images

	Western blotting analysis using anti-calcium binding protein 39 antibody (Cat#61819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calcium binding protein 39 antibody (Cat#61819, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-calcium binding protein 39 antibody (Cat#61819). Calcium binding protein 39 expression in wild-type (WT) and calcium binding protein 39 (CAB39) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-calcium binding protein 39 antibody (Cat#61819, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Calcium binding protein 39 expression in HepG2 cells using anti-Calcium binding protein 39 antibody (Cat#61819, 1:2,000). Green, isotype control; red, Calcium binding protein 39.
	Immunocytochemical staining of HepG2 cells with anti-Calcium binding protein 39 antibody (Cat#61819, 1:1,000). Nuclei were stained blue with DAPI; Calcium binding protein 39 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.