Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	cAMP responsive element binding protein 1
Gene Symbol:	CREB1
Synonyms:	CREB; CREB-1
Gene ID:	1385
UniProt ID:	P16220
Clone ID:	25GB3650
Immunogen:	A synthesized peptide derived from human CREB
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene CREB1 encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-CREB1 antibody (Cat#61764). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#61764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-CREB1 antibody (Cat#61764). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#61764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CREB1 expression in HepG2 cells using anti-CREB1 antibody (Cat#61764, 1:2,000). Green, isotype control; red, CREB1.
	Immunocytochemical staining of HepG2 cells with anti-CREB1 antibody (Cat#61764, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.