Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	SUMO specific peptidase 1
Gene Symbol:	SENP1
Synonyms:	SuPr-2
Gene ID:	29843
UniProt ID:	Q9P0U3
Clone ID:	25GB3580
Immunogen:	A synthesized peptide derived from human SENP1
Dilution:	WB 1:4,000-1:20,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-SUMO specific peptidase 1 antibody (Cat#61754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-SUMO specific peptidase 1 antibody (Cat#61754). SUMO specific peptidase 1 expression in wild-type (WT) and SUMO specific peptidase 1 (SENP1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of SUMO specific peptidase 1 expression in HepG2 cells using anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:2,000). Green, isotype control; red, SUMO specific peptidase 1.