Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	vimentin
Gene Symbol:	VIM
Gene ID:	7431
UniProt ID:	P08670
Clone ID:	25GB3480
Immunogen:	A synthesized peptide derived from human Phospho-Vimentin (Ser72)
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Images

	Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#61732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#61732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#61732). Phospho-vimentin (Ser72) expression in wild-type (WT) and VIM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#61732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using anti-Phospho-Vimentin (Ser72) antibody (Cat#61732, 1:2,000). Green, isotype control; red, Phospho-Vimentin (Ser72).
	Immunocytochemical staining of HT-1080 cells with anti-Phospho-Vimentin (Ser72) antibody (Cat#61732, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.