Description

Reactivity:	Human, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	CASP2 and RIPK1 domain containing adaptor with death domain
Gene Symbol:	CRADD
Synonyms:	MRT34; RAIDD
Gene ID:	8738
UniProt ID:	P78560
Clone ID:	25GB3380
Immunogen:	A synthesized peptide derived from human RAIDD
Dilution:	WB 1:4,000-1:20,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with cognitive disability. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-CRADD antibody (Cat#61701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRADD antibody (Cat#61701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-CRADD antibody (Cat#61701). CRADD expression in wild type (WT) and CRADD knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CRADD antibody (Cat#61701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of CRADD expression in HepG2 cells using anti-CRADD antibody (Cat#61701, 1:2,000). Green, isotype control; red, CRADD.