Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	transcription factor 7 like 2
Gene Symbol:	TCF7L2
Synonyms:	TCF4; TCF-4
Gene ID:	6934
UniProt ID:	Q9NQB0
Clone ID:	25GB3275
Immunogen:	A synthesized peptide derived from human TCF7L2
Dilution:	WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-transcription factor 7 like 2 antibody (Cat#61671). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-transcription factor 7 like 2 antibody (Cat#61671, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-transcription factor 7 like 2 antibody (Cat#61671). Transcription factor 7 like 2 expression in wild-type (WT) and transcription factor 7 like 2 (TCF7L2) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-transcription factor 7 like 2 antibody (Cat#61671, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Transcription factor 7 like 2 expression in HepG2 cells using anti-Transcription factor 7 like 2 antibody (Cat#61671, 1:2,000). Green, isotype control; red, Transcription factor 7 like 2.
	Immunocytochemical staining of HepG2 cells with anti-Transcription factor 7 like 2 antibody (Cat#61671, 1:1,000). Nuclei were stained blue with DAPI; Transcription factor 7 like 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.