Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	RB transcriptional corepressor 1
Gene Symbol:	RB1
Synonyms:	RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1
Gene ID:	5925
UniProt ID:	P06400
Clone ID:	25GB3245
Immunogen:	A synthesized peptide derived from human Phospho-Retinoblastoma (S807)
Dilution:	WB 1:4,000-1:20,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by the gene RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Images

	Western blotting analysis using anti-phospho-retinoblastoma (S807) antibody (Cat#61667). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-retinoblastoma (S807) antibody (Cat#61667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-retinoblastoma (S807) antibody (Cat#61667). Phospho-retinoblastoma (S807) expression in wild-type (WT) and RB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-retinoblastoma (S807) antibody (Cat#61667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Phospho-Retinoblastoma (S807) expression in HAP-1 cells using anti-Phospho-Retinoblastoma (S807) antibody (Cat#61667, 1:2,000). Green, isotype control; red, Phospho-Retinoblastoma (S807).