Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	eukaryotic translation initiation factor 4E
Gene Symbol:	EIF4E
Synonyms:	CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
Gene ID:	1977
UniProt ID:	P06730
Clone ID:	25GB3235
Immunogen:	A synthesized peptide derived from human Phospho-eIF4E (S209)
Dilution:	WB 1:2,000-1:10,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5′ end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5′-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Phospho-eIF4E (S209) expression in wild type (WT) and EIF4E knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Phospho-eIF4E (S209) expression in C2C12 cells using anti-Phospho-eIF4E (S209) antibody (Cat#61666, 1:2,000). Green, isotype control; red, Phospho-eIF4E (S209).
	Immunocytochemical staining of C2C12 cells with Phospho-eIF4E (S209) antibody (Cat#61666, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4E (S209) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.