Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	adenosine deaminase RNA specific
Gene Symbol:	ADAR
Synonyms:	DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
Gene ID:	103
UniProt ID:	P55265
Clone ID:	25GB3090
Immunogen:	A synthesized peptide derived from human ADAR1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The gene ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#61635). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#61635, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#61635). Adenosine deaminase RNA specific expression in wild-type (WT) and adenosine deaminase RNA specific (ADAR) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#61635, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#61635, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific.
	lmmunocytochemical staining of HepG2 cells with Adenosine deaminase RNA specific antibody (Cat#61635, 1:1,000). Nuclei were stained blue with DAPl; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.