Background

This gene CAPG encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.

Images

	Western blotting analysis using anti-CAPG antibody (Cat#61619). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-CAPG antibody (Cat#61619). CAPG expression in wild-type (WT) and CAPG knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CAPG expression in C2C12 cells using anti-CAPG antibody (Cat#61619, 1:2,000). Green, isotype control; red, CAPG.
	Immunocytochemical staining of C2C12 cells with anti-CAPG antibody (Cat#61619, 1:1,000). Nuclei were stained blue with DAPI; CAPG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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