Description

Reactivity:	Human, Mouse
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	mitogen-activated protein kinase kinase 2
Gene Symbol:	MAP2K2
Synonyms:	CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Gene ID:	5605
UniProt ID:	P36507
Clone ID:	25GB2935
Immunogen:	A synthesized peptide derived from human MEK2
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Images

	Western blotting analysis using anti-MAP2K2 antibody (Cat#61587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K2 antibody (Cat#61587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-MAP2K2 antibody (Cat#61587). MAP2K2 expression in wild-type (WT) and MAP2K2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K2 antibody (Cat#61587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of MAP2K2 expression in HepG2 cells using anti-MAP2K2 antibody (Cat#61587, 1:2,000). Green, isotype control; red, MAP2K2.
	Immunocytochemical staining of HepG2 cells with anti-MAP2K2 antibody (Cat#61587, 1:1,000). Nuclei were stained blue with DAPI; MAP2K2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.