Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	chromobox 5
Gene Symbol:	CBX5
Synonyms:	HP1; HP1A; HEL25; HP1alpha
Gene ID:	23468
UniProt ID:	P45973
Clone ID:	25GB2865
Immunogen:	A synthesized peptide derived from human HP1 alpha
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Images

	Western blotting analysis using anti-chromobox 5 antibody (Cat#61562). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 5 antibody (Cat#61562, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-chromobox 5 antibody (Cat#61562). Chromobox 5 expression in wild-type (WT) and chromobox 5 (CBX5) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 5 antibody (Cat#61562, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#61562, 1:2,000). Green, isotype control; red, Chromobox 5.
	Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#61562, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.