Description

Reactivity:	Human, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	cysteinyl-tRNA synthetase 1
Gene Symbol:	CARS1
Synonyms:	CARS; MDBH; CYSRS; MCDDBH; MGC:11246
Gene ID:	833
UniProt ID:	P49589
Clone ID:	25GB2790
Immunogen:	A synthesized peptide derived from human CARS
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene CARS1 encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.

Images

	Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550). Cysteinyl-tRNA synthetase 1 expression in wild-type (WT) and cysteinyl-tRNA synthetase 1 (CARS1) knockdown (KD) HSHC cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Cysteinyl-tRNA synthetase 1 expression in HT-1080 cells using anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:2,000). Green, isotype control; red, Cysteinyl-tRNA synthetase 1.
	Immunocytochemical staining of HT-1080 cells with anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:1,000). Nuclei were stained blue with DAPI; Cysteinyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.