Description

Reactivity:	Human, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	tripartite motif containing 25
Gene Symbol:	TRIM25
Synonyms:	EFP; Z147; RNF147; ZNF147
Gene ID:	7706
UniProt ID:	Q14258
Clone ID:	25GB2695
Immunogen:	A synthesized peptide derived from human TRIM25
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.

Images

	Western blotting analysis using anti-TRIM25 antibody (Cat#61535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM25 antibody (Cat#61535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-TRIM25 antibody (Cat#61535). TRIM25 expression in wild-type (WT) and TRIM25 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM25 antibody (Cat#61535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of TRIM25 expression in HepG2 cells using anti-TRIM25 antibody (Cat#61535, 1:2,000). Green, isotype control; red, TRIM25.
	Immunocytochemical staining of HepG2 cells with anti-TRIM25 antibody (Cat#61535, 1:1,000). Nuclei were stained blue with DAPI; TRIM25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.