Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	histone deacetylase 8
Gene Symbol:	HDAC8
Synonyms:	HD8; WTS; RPD3; CDA07; CDLS5; KDAC8; MRXS6; HDACL1
Gene ID:	55869
UniProt ID:	Q9BY41
Clone ID:	25GB2495
Immunogen:	A synthesized peptide derived from human HDAC8
Dilution:	WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Histone deacetylase 8 expression in wild-type (WT) and histone deacetylase 8 (HDAC8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Histone deacetylase 8 expression in HeLa cells using Histone deacetylase 8 antibody (Cat#61423, 1:2,000). Green, isotype control; red, Histone deacetylase 8.
	Immunocytochemical staining of HeLa cells with anti-Histone deacetylase 8 antibody (Cat#61423, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.