Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	mannose receptor C-type 1
Gene Symbol:	MRC1
Synonyms:	MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Gene ID:	4360
UniProt ID:	P22897
Clone ID:	25GB2060
Immunogen:	A synthesized peptide derived from human MRC1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Images

	Western blotting analysis using anti-mannose receptor (CD206) antibody (Cat#61279). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mannose receptor (CD206) antibody (Cat#61279, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-mannose receptor (CD206) antibody (Cat#61279). Mannose receptor (CD206) expression in wild-type (WT) and MRC1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-mannose receptor (CD206) antibody (Cat#61279, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Mannose Receptor (CD206) expression in HepG2 cells using Mannose Receptor (CD206) antibody (Cat#61279, 1:2,000). Green, isotype control; red, Mannose Receptor (CD206).
	lmmunocytochemical staining of HepG2 cells with Mannose Receptor (CD206) antibody (Cat#61279, 1:1,000). Nuclei were stained blue with DAPl; Mannose Receptor (CD206) was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.