Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	kelch like ECH associated protein 1
Gene Symbol:	KEAP1
Synonyms:	INrf2; KLHL19
Gene ID:	9817
UniProt ID:	Q14145
Clone ID:	25GB495
Immunogen:	Recombinant protein of human KEAP1
Dilution:	WB 1:2,500-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Images

	Western blotting analysis using anti-KEAP1 antibody (Cat#61257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KEAP1 antibody (Cat#61257, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-KEAP1 antibody (Cat#61257). KEAP1 expression in wild-type (WT) and KEAP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-KEAP1 antibody (Cat#61257, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of KEAP1 expression in HAP-1 cells using anti-KEAP1 antibody (Cat# 61257, 1:2,000). Green, isotype control; red, KEAP1.