Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | vesicle associated membrane protein 8 |
| Gene Symbol: | VAMP8 |
| Synonyms: | EDB; VAMP-8 |
| Gene ID: | 8673 |
| UniProt ID: | Q9BV40 |
| Clone ID: | 25GB590 |
| Immunogen: | A synthesized peptide derived from human VAMP8 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.
Images
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Western blotting analysis using anti-vesicle associated membrane protein 8 antibody (Cat#61255). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vesicle associated membrane protein 8 antibody (Cat#61255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-vesicle associated membrane protein 8 antibody (Cat#61255). Vesicle associated membrane protein 8 expression in wild-type (WT) and vesicle associated membrane protein 8 (VAMP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-vesicle associated membrane protein 8 antibody (Cat#61255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Vesicle associated membrane protein 8 expression in HepG2 cells using anti-Vesicle associated membrane protein 8 antibody (Cat# 61255, 1:2,000). Green, isotype control; red, Vesicle associated membrane protein 8. |
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