Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	receptor for activated C kinase 1
Gene Symbol:	RACK1
Synonyms:	H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
Gene ID:	10399
UniProt ID:	P63244
Clone ID:	23GB2355
Immunogen:	A synthesized peptide derived from human RACK1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including regulation of protein metabolic process; regulation of signal transduction; and regulation of vesicle-mediated transport. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex.

Images

	Western blotting analysis using anti-RACK1 antibody (Cat#61248). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RACK1 antibody (Cat#61248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-RACK1 antibody (Cat#61248). RACK1 expression in wild-type (WT) and RACK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RACK1 antibody (Cat#61248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of RACK1 expression in HepG2 cells using RACK1 antibody (Cat#61248, 1:2,000). Green, isotype control; red, RACK1.
	Immunocytochemical staining of HepG2 cells with RACK1 antibody (Cat#61248, 1:1,000). Nuclei were stained blue with DAPI; RACK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.