Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.

Images

	Western blotting analysis using anti-Interferon regulatory factor 3 antibody (Cat#61219). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Interferon regulatory factor 3 antibody (Cat#61219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-interferon regulatory factor 3 antibody (Cat#61219). Interferon regulatory factor 3 expression in wild type (WT) and interferon regulatory factor 3 (IRF3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon regulatory factor 3 antibody (Cat#61219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Interferon regulatory factor 3 expression in HepG2 cells using anti-Interferon regulatory factor 3 antibody (Cat# 61219, 1:2,000). Green, isotype control; red, Interferon regulatory factor 3.

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