Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | O-GlcNAcase |
| Gene Symbol: | OGA |
| Synonyms: | MEA5; MGEA5; NCOAT |
| Gene ID: | 10724 |
| UniProt ID: | O60502 |
| Clone ID: | 24GB6120 |
| Immunogen: | A synthesized peptide derived from human MGEA5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).
Images
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Western blotting analysis using anti-MGEA5 antibody (Cat#61204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MGEA5 antibody (Cat#61204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-MGEA5 antibody (Cat#61204). MGEA5 expression in wild-type (WT) and OGA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MGEA5 antibody (Cat#61204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). OGA, O-GlcNAcase. |
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Flow cytometric analysis of MGEA5 expression in HAP-1 cells using MGEA5 antibody (Cat#61204, 1:2,000). Green, isotype control; red, MGEA5. |
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Immunocytochemical staining of HAP-1 cells with MGEA5 antibody (Cat#61204, 1:1,000). Nuclei were stained blue with DAPI; MGEA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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