Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	lysyl-tRNA synthetase 1
Gene Symbol:	KARS1
Synonyms:	KRS; KARS; KARS2; LEPID; CMTRIB; DEAPLE; DFNB89
Gene ID:	3735
UniProt ID:	Q15046
Clone ID:	24GB6130
Immunogen:	A synthesized peptide derived from human LysRS
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-lysyl-tRNA synthetase 1 antibody (Cat#61196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-lysyl-tRNA synthetase 1 antibody (Cat#61196). Lysyl-tRNA synthetase 1 expression in wild-type (WT) and lysyl-tRNA synthetase 1 (KARS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of lysyl-tRNA synthetase 1 expression in HepG2 cells using lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:2,000). Green, isotype control; red, lysyl-tRNA synthetase 1.
	Immunocytochemical staining of HepG2 cells with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:1,000). Nuclei were stained blue with DAPI; Lysyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.