Description

Reactivity:	Human, Mouse
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	inhibin subunit beta B
Gene Symbol:	INHBB
Gene ID:	3625
UniProt ID:	P09529
Clone ID:	24GB6100
Immunogen:	A synthesized peptide derived from human Inhibin beta B
Dilution:	WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of the dimeric activin and inhibin protein complexes. These complexes activate and inhibit, respectively, follicle stimulating hormone secretion from the pituitary gland. Polymorphisms near this gene are associated with pre-eclampsia in female human patients.

Images

	Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Green, isotype control; red, inhibin subunit beta B.
	Immunocytochemical staining of HT-1080 cells with anti-inhibin subunit beta B antibody (Cat#61184, 1:1,000). Nuclei were stained blue with DAPI; Inhibin subunit beta B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.