Background

The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-histone deacetylase 10 antibody (Cat#61171). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 10 antibody (Cat#61171, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-histone deacetylase 10 antibody (Cat#61171). Histone deacetylase 10 expression in wild-type (WT) and histone deacetylase 10 (HDAC10) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-histone deacetylase 10 antibody (Cat#61171, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of histone deacetylase 10 expression in HepG2 cells using histone deacetylase 10 antibody (Cat#61171, 1:2,000). Green, isotype control; red, histone deacetylase 10.
	Immunocytochemical staining of HepG2 cells with anti-histone deacetylase 10 antibody (Cat#61171, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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